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KMID : 0624620160490020116
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BMB Reports
2016 Volume.49 No. 2 p.116 ~ p.121
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Mitochondrial oxidative phosphorylation complexes exist in the sarcolemma of skeletal muscle
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Lee Hyun
Kim Seung-Hyeob
Lee Jae-Seon
Yang Yun-Hee
Nam Jwa-Min
Kim Bong-Woo
Ko Young-Gyu
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Abstract
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Although proteomic analyses have revealed the presence of mitochondrial oxidative phosphorylation (OXPHOS) proteins in the plasma membrane, there have been no in-depth evaluations of the presence or function of OXPHOS I-V in the plasma membrane. Here, we demonstrate the in situ localization of OXPHOS I-V complexes to the sarcolemma of skeletal muscle by immunofluorescence and immunohistochemistry. A portion of the OXPHOS I-V complex proteins was not co-stained with MitoTracker but co-localized with caveolin-3 in the sarcolemma of mouse gastrocnemius. Mitochondrial matrix-facing OXPHOS complex subunits were ectopically expressed in the sarcolemma of the non-permeabilized muscle fibers and C2C12 myotubes. The sarcolemmal localization of cytochrome c was also observed from mouse gastrocnemius muscles and C2C12 myotubes, as determined by confocal and total internal resonance fluorescence (TIRF) microscopy. Based on these data, we conclude that a portion of OXPHOS complexes is localized in the sarcolemma of skeletal muscle and may have non-canonical functions.
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KEYWORD
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Cytochrome c, Extracellular NADH-dependent respiration, Oxidative phosphorylation complexes, Sarcolemma, Skeletal muscle
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